Secondary metabolic profiling, antioxidant potential, enzyme inhibitory activities and in silico and ADME studies: a multifunctional approach to reveal medicinal and industrial potential of Tanacetum falconeri

Tanacetum falconeri is a significant flowering plant that possesses cytotoxic, insecticidal, antibacterial, and phytotoxic properties. Its chemodiversity and bioactivities, however, have not been thoroughly investigated. In this work, several extracts from various parts of T. falconeri were assessed for their chemical profile, antioxidant activity, and potential for enzyme inhibition. The total phenolic contents of T. falconeri varied from 40.28 ± 0.47 mg GAE/g to 11.92 ± 0.22 mg GAE/g in various extracts, while flavonoid contents were found highest in TFFM (36.79 ± 0.36 mg QE/g extract) and lowest (11.08 ± 0.22 mg QE/g extract) in TFSC (chloroform extract of stem) in similar pattern as found in total phenolic contents. Highest DPPH inhibition was observed for TFFC (49.58 ± 0.11 mg TE/g extract) and TFSM (46.33 ± 0.10 mg TE/g extract), whereas, TFSM was also potentially active against (98.95 ± 0.57 mg TE/g) ABTS radical. In addition, TFSM was also most active in metal reducing assays: CUPRAC (151.76 ± 1.59 mg TE/g extract) and FRAP (101.30 ± 0.32 mg TE/g extract). In phosphomolybdenum assay, the highest activity was found for TFFE (1.71 ± 0.03 mg TE/g extract), TFSM (1.64 ± 0.035 mg TE/g extract), TFSH (1.60 ± 0.033 mg TE/g extract) and TFFH (1.58 ± 0.08 mg TE/g extract), while highest metal chelating activity was recorded for TFSH (25.93 ± 0.79 mg EDTAE/g extract), TFSE (22.90 ± 1.12 mg EDTAE/g extract) and TFSC (19.31 ± 0.50 mg EDTAE/g extract). In biological screening, all extracts had stronger inhibitory capacity against AChE while in case of BChE the chloroform extract of flower (TFFC) and stem (TFSC) showed the highest activities with inhibitory values of 2.57 ± 0.24 and 2.10 ± 0.18 respectively. Similarly, TFFC and TFSC had stronger inhibitory capacity (1.09 ± 0.015 and 1.08 ± 0.002 mmol ACAE/g extract) against α-Amylase and (0.50 ± 0.02 and 0.55 ± 0.02 mmol ACAE/g extract) α-Glucosidase. UHPLC-MS study of methanolic extract revealed the presence of 133 components including sterols, triterpenes, flavonoids, alkaloids, and coumarins. The total phenolic contents were substantially linked with all antioxidant assays in multivariate analysis. These findings were validated by docking investigations, which revealed that the selected compounds exhibited high binding free energy with the enzymes tested. Finally, it was found that T. falconeri is a viable industrial crop with potential use in the production of functional goods and nutraceuticals.


Introduction
Tanacetum falconeri is an important flowering herb belong to plant family Asteraceae [1].It is mostly found in the rocky talus, near the lakes, valley plains or grassy ridges in different parts of Pakistan.Locally, the powdered leaves and extract of leaves of T. falconeri is used against various abdominal problems, whereas, its flowers and buds are beneficial in treating asthma, jaundice and blood pressure problems [2,3].Different plant parts are utilised to treat joint discomfort after being dried in the shade [4].The habitants of Kallaway Indians and the Andes mountains used these plants for back ache, abdominal pain and gastric trouble [5].The Mexican people used it as a tonic to regulate menstruation and as an antispasmodic.In Venezuela, it's used to cure earaches [6].A scanty work on chemodiversity and biological potential of T. falconeri has been reported in literature, however, other Tanacetum plants are rich in terpenes mostly as essential oils [7][8][9][10][11][12][13], sterols [14][15][16], phenolic acids and flavonoids [17].Due to the presence of variety of bioactive compounds, Tanacetum plants extracts have shown various biological activities like anti-inflammatory, antiviral, antifungal, antibacterial and antioxidant [18], edema [19], antibacterial [14,20], fungicidal activity [21], antioxidant [22], anti-inflammatory [23], anthelmintic, Anticoagulant and antifibrinolytic, insecticidal [14], and anti-ulcer [24,25] and antitumor [26].Tanacetum plants have also showed anti-Leishmanial, antibacterial [27], antimalarial [28] activities.Despite of the lack of phytochemical investigation, Tanacetum plants has received recognition as a potential nematocidal, insecticidal, antibacterial, cytotoxic, and phytotoxic herbs [29].Therefore, the diverse chemical profiling of Tanacetum plants, and their medicinal uses prompted us to investigate T. falconeri for its chemodiversity and biological potential.The goal of this study was to evaluate the traditional therapeutic applications of T. falconeri by evaluating the various extracts for their total bioactive content, full secondary metabolic profile, and bioactivities.In vitro tests were conducted to evaluate the anti-oxidant (DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, ferrous chelating) and enzyme inhibitory capabilities of all extracts against various enzymes linked to skin, neurodegenerative, and diabetic illnesses.Additionally, multivariate analysis and docking investigations were carried out.

Collection of the plant material and identification
The plant material was collected from Shigar District, Gilgit-Baltistan, Pakistan and was identified by Dr. Zaheer Abbas, a taxonomist at the University of Education, DG Khan Campus, Dera Ghazi Khan, where a voucher specimen No. BT-0063 has been deposited in the herbarium of same university.

Preparation of the extracts
The collected plant material was divided into flower (TFF) and stem with leaves (TFS) parts, which were then dried under shade for one week.Each part (600 and 800 g, respectively) was divided into four parts, which were then extracted separately through maceration using n-hexane, chloroform, ethyl acetate and methanol to get crude extracts of the stem: TFSM: methanolic extract of stem; TFSH: hexane extract of stem; TFSE: ethyl acetate extract of stem; TFSC: chloroform extract of stem and flowers extracts: TFFM: methanolic extract of flowers; TFFH: hexane extract of flowers; TFFE: ethyl acetate extract of flowers; TFFC: chloroform extract of flowers.All these extracts were then studied for their phenolic and flavonoid contents, antioxidant and enzyme inhibition studies and chemodiversity.

Estimation of Total phenolic (TPC) and Total flavonoid (TFC) contents
The Estimation of total phenolic (TPC) and total flavonoid (TFC) contents were done through same methods as we reported previously [30][31][32][33].The results of total phenolic contents (TPC) were presented in milligrams of gallic acid equivalent per grams of extract (mg GAE/g extract).The total flavonoid contents (TFC) results were reported in milligrams of rutin equivalent per grams of extract (mg RE/g extract).

Antioxidant activities assays
The antioxidant activities of extracts were measured by following pre-established protocols as we reported previously [30][31][32][33].For FRAP, ABTS, DPPH, CUPRAC, and total antioxidant capacity, trolox equivalent was utilized as standard and results were expressed as mg TE/g extract; while for metal chelating assays, ethylene diamine tetraacetic acid (EDTA) was the standard and results were expressed as mg TE/g extract.

Enzyme inhibition assays
The α-amylase, α-glucosidase, BChE, tyrosinase, and AChE enzyme inhibitory assays were conducted using previously published methods [30][31][32][33].Acarbose (mmol ACAE/g extract) was used as a standard to measure the inhibitory activity of α-amylase and α-glucosidase.Galantamine (mg GALAE/g extract) was used to measure the inhibitory activity of AChE and BChE, and kojic acid (mmol KAE/g extract) was used to measure the inhibitory activity of tyrosinase.

UHPLC-MS analysis
UHPLC-MS (ultra-high performance liquid chromatography mass spectrometry) analysis was used to profile secondary metabolites using an Agilent 1290 Infinity UHPLC system coupled to an Agilent 6520 Accurate-Mass Q-TOF mass spectrometer with dual ESI source, as we previously reported [30][31][32].The column was an Agilent Zorbax Eclipse XDB-C18 with 3.5 m in thickness and 2.1 × 150 mm in diameter.A 0.1% formic acid solution in water served as mobile phase A, while a 0.1% formic acid solution in acetonitrile served as mobile phase B. A consistent flow rate of 0.5 millilitres per minute was maintained.One microliter of methanolic extract was given for twenty-five minutes, and then there was a fiveminute post-run period.The secondary metabolites were found using the METLIN database.

Statistical analysis
The experiments were performed in triplicate, and differences between the extracts were compared using an ANOVA and Tukey's test.Pearson correlation analysis was used to establish the link between total bioactive components and biological activity assays.Graph Pad Prism (version 9.2) was used for the analysis.To assess the degree of similarity or difference between the extracts, a PCA was carried out using SIMCA (version 14.0).

Docking study methodology
The chemical structures of the five enzymes with the highest resolution were downloaded from the protein data bank in PDB format.Discovery Studio (DS 2021Client) software was employed to formulate protein molecules.Attached chemical moieties (water molecules and other ligand) were removed from macromolecules.Afterward, they were transferred onto the PyRx program (version 0.8) for docking purposes in pdbqt file that contains a protein structure with hydrogens in all polar residues.The structures of selected ligands were acquired from the Pubchem as 3D SDF formats.The software specification and procedure of docking were followed as described by Ahmed et al., [34].The enzyme molecules were loaded into PyRx and converted to macromolecules by using autodock embedded in PyRx software.Then the ligands were attached using the open babel tool, and energy was minimized to obtain the stable structure; then, ligands were converted to pdbqt format.The docking site on the protein target was defined by establishing a grid box, which was maximized using "maximize" option for better coverage of active site and exhaustiveness was 8.The other settings of the software were used as "default".The best conformation with the lowest docked energy was chosen after the docking search was completed.The molecular docking result for each compound was visualized as an output pdbqt file by using the molecular graphics laboratory (mgltool) tool.Interactions were finally visualized in discovery studio by using mgltool, to determine some specific contacts between the atoms of the test compounds and amino acids residues of the studied protein molecules [35].

Total phenolic (TPC) and flavonoid (TFC) contents of T. falconeri
Phenolic compounds are important component of nutraceuticals and functional foods because of their antioxidant properties.The antioxidant properties of phenolics are usually attributed to the presence of hydroxyl group(s) on the benzene ring, which goes about an electron donor and consequently and straight forwardly includes in quenching free radicals.In the present study, several solvent-based crude stem and flower extracts of T. falconeri were screened for their total phenolic and flavonoid contents.Total phenolic contents (TPC) observed in the methanol extract of flower (TFFM) were high (40.28 ± 0.47 mg GAE/g extract), followed by the TFFH extract (33.00 ± 0.67 mg GAE/g extract).Although the same trend of TPC for stem extracts was seen in TFSM (22.21 ± 0.17 mg GAE/g extract) and TFSH (24.34 ± 0.49 mg GAE/g extract) but were lower than those of respective extracts of the flower.Ethyl acetate extracts from both the sources were next in line (Table 1).Similarly, total flavonoids contents (TFC) were also observed high in flower extracts (TFFM 36.79 ± 0.36 mg QE/g extract and TFFH 32.80 ± 0.80 mg QE/g extract), followed by stem extracts (TFSM 17.68 ± 0.32 mg QE/g extract and TFSE 23.38 ± 0.17 mg QE/g extract).In both the cases, lowest phenolic and flavonoid contents were calculated in chloroform extracts (Table 1).Usually phenolics and flavonoids are relatively polar compounds; therefore, their high concentration in methanolic or ethyl acetate extracts seems reasonable, however, in case of flower extracts (TFFH and TFSH), the high amount of phenolic contents could be attributed to the possible presence of esters of phenolic acids in the extracts.Literature reports also substantiate our findings where methanolic extracts of Tanacetum plants have been reported to be rich in phenolic contents [36].Another report describes that Tanacetum species produce high level of vanillic acid, and caffeic acid, catechin and quercetin [37] and other phenolics and flavonoids [38,39].;The presence of caffeoylquinic acids in Tanacetum species [40,41] substantiates our deduction that phenolic acid esters are present in T. falconeri, which are extracted in low polar solvents, and thus TFFH and TFSH also afforded high amount of phenolic contents.

Antioxidant activities of the extracts of T. falconeri
Research showed that the antioxidant activity of a plant extract is usually associated to the phenolic contents, i.e. higher the phenolic contents, higher will be the activity [42].However, in the present study, the highest DPPH free radical scavenging activity (TFFM; 49.58 ± 0.11 mg TE/g extract) was associated to the methanolic flower extract, which is followed by n-hexane flower extract (TFFH; 47.91 ± 0.17 mg TE/g extract), whereas, methanolic stem extract (TFSM) also showed nearly similar inhibition (43.75 ± 0.41 mg TE/g extract).
It is already predicted that the presence of phenolic contents in low polar solvents could be of the nature of phenolic acid esters.Literature search revealed that phenolic acid esters are potent antioxidants [43], therefore, the activity of TFFM could be attributed to such kind of compounds and other metabolites.On the other hand, the higher DPPH free radical inhibitory potential of TFSM could be attested for its high phenolic contents (Table 2).TFSM and TFFH were also significantly active, while other extracts were found inactive (Table 2).In case of ABTS free radical activity same pattern was observed as in DPPH and TFFM exhibited highest inhibition value (112.61 ± 0.15 mg TE/g extract).The next in line were TFFH, TFSM and TFSE (Table 2) with values of 84.60 ± 0.57, 73.43 ± 2.77 and 62.51 ± 0.97 respectively.In metal ion reducing power assays, again the TFFM was highly active (CUPRAC: 160.48 ± 6.59 mg TE/g extract; FRAP: 102.58 ± 2.62 mg TE/g extract), followed by the methanolic extract of stem (TFSM).All other extracts also exhibited significant and comparable metal reducing power (Table 2).TFSE was most active in phosphomolybdenum with the value of 1.71 ± 0.03 mg TE/g extract, whereas, TFFH and TFSH were also significantly active with the values of 1.64 ± 0.00 and 1.58 ± 0.08 mg TE/g extract, Table 1 Total bioactive contents of T. falconeri   2).It if further noticed that more polar extracts were also weak chelators, however, overall the present study revealed that T. falconeri is a potential antioxidant plant to be considered for its uses in health promoting formulations.

Enzyme inhibition activities of the extracts of T. falconeri AChE and BChE enzyme inhibition activities
Alzheimer's disease (AD), a noncommunicable disease (NCDs) has been identified as a largely increasing health challenge worldwide.It is an irreversible, progressive form of dementia, associated with an ongoing decline of brain functioning [44] and thus causes memory loss.The World Health Organization (WHO) has reported that more than 30 million people are afflicted by AD and this number is expected to become double every two decades to reach ~ 115 million by 2050.This problem is thus expected to weaken the social and economic development and may affect the social services [45].Acetylcholine (ACh) and buytrylcholine (BCh) are important neurotransmitters requires for proper brain, memory and body functioning Therefore, low levels of cholines lead to memory issues and muscle disorders.Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are the enzymes that hydrolyse acetycholine and butyrylcholine, respectively [46].The inhibitors of these two enzymes results into accumulation of the neurotransmitter acetylcholine and enhanced stimulation of postsynaptic cholinergic receptors [47,48].Natural products have already proven to be promising sources of useful acetylcholinesterase (AChE) inhibitors [49].The currently approved drugs for AD, galantamine and rivastigmine, are plantderived alkaloids, which offer symptomatic relief from AD [50].These facts suggest to investigate the use of medicinal plants and their formulations to prevent and treat neurodegenerative disease [51].

Tyrosinase enzyme inhibition activities
Browning of raw food and hyperpigmentation of human skin are two undesirable processes caused a group of copper-containing enzyme tyrosinase (EC 1.14.18.1).Hyper activity of tyrosinase causes results in a less attractive appearance and loss in nutritional quality of food and blackening of human skin [52].Further, over production of melanin in human skin causes several skin disorders such as melasma, senile lentigines and freckles and thus exert a considerable psychosomatic effect on affected patients [53].These problems can be controlled by using tyrosinase inhibitors.Presently available tyrosinase inhibitors like hydroquinone, arbutin, kojic acid, ascorbic acid, ellagic acid and others have different problems either in their use or the bioavailability [54].Therefore, there is a great need to discover and develop new but safer tyrosinase inhibitors.For his purpose, the medicinal plant extracts are the main agents being researched and used as tyrosinase inhibitors in these days.In the present work various extracts of T. falconeri were evaluated for their tyrosinase inhibitory activity.Methanolic extracts of both flowers (TFFM) and stem TFSM) of T. falconeri were the most active with inhibition values as 35.53 ± 0.35 and 35.30 ± 0.70 mg KAE/g extract, respectively followed by the hexane extracts (29.96 ± 0.10 and 32.41 ± 1.91 mg KAE/g extract, respectively.All other extracts exhibited equal but significant inhibitory potential (Table 3), which disclosed that T. falconeri can be a potential ingredient in cosmetic and food industry.

α-Amylase and α-glucosidase enzyme inhibition activities
Diabetes mellitus is another major non-communicable metabolic disease that has high impact on health and economy.A published report revealed that only in 2014, 4.9 million deaths were recorded due to diabetes [55].In diabetic patients usually the blood glucose level increases after taking meal and thus causes postprandial hyperglycemia [56].The glycosidic linkage in carbohydrates is broken by α-amylase to produce oligosaccharides, which are then degraded to glucose by α-glucosidase [57].Since both the enzymes digest the carbohydrates and cause diabetes [55]; inhibition of the activity of these enzymes can delay the increase in blood glucose level and reduce the risk of developing diabetes [58].Among current inhibitors, only acarbose inhibits both α-amylase and α-glucosidase, whereas, miglitol and voglibose inhibit only α-glucosidase [59,60].Literature search revealed that some of the plant extracts or pure phytochemicals were found effective against both enzymes [61][62][63][64], which leads to conclude that medicinal plants can serve as potential antidiabetic agents.Therefore, in the present study, the flower and stem extracts of T. falconeri were evaluated for their inhibitory potential against α-amylase and α-glucosidase.Against amylase, all the extracts exhibited significant activity with inhibitory values in the range of 0.38 ± 0.009 to 0.55 ± 0.002 mg ACAE/g extract, while against α-glucosidase the inhibitory values were observed between 0.46 ± 0.002 to 1.09 ± 0.015 mg ACAE/g extract, with lowest potential in both the cases was observed for methanolic extracts (Table 3).It is reported that the plant extracts exhibit antidiabetic properties due to the combined effect of biologically active compounds like polyphenols, carotenoids, lignans, coumarins, glucosinolates, etc. [65].These plant metabolites as a combined effect, improve the performance of pancreatic tissue by increasing insulin secretions or by reducing the intestinal absorption of glucose [66].Therefore, the anti-amylase and anti-glucosidase activities of the extracts of T. falconeri could be attributed to the presence of such metabolites.Literature reports revealed that most of the plant extracts and pure compounds exhibit selective inhibition against either α-amylase or α-glucosidase; while only fewer have been found active against both the enzymes [55].Overall the crude extracts of T. falconeri were found significantly active against both the enzymes; therefore, it can be a potential component of crude antidiabetic drugs.

UHPLC-MS Analysis
UHPLC-MS Analysis of methanolic extract (Fig. 1) of flowers result in identification of 133 compounds (Table 4) of various secondary metabolites class mainly phenolic, flavonoids, Alkaloids, terpenoids and steroids.The presence of important phenolic and flavonoids compounds like 6-Caffeoylsucrose, 3-O-Feruloylquinic acid, Brosimacutin C, Quinic acid, Rutacultin, Castamollissin, Kaempferol 3-p-coumarate and Methylsyringin may be responsible for antioxidant activities [67].These results demonstrated that T. falconeri is not limited to a specific class of secondary metabolites and can create a broad variety of compounds.Chemodiversity makes T. falconeri a valuable herb with a broad range of bioactivities.

Data analysis
Multivariate analysis provides a bridge between diverse parameters and their interactions.This makes it a fundamental tool in phytochemical studies to gain more information on the relationship between the chemical       For this purpose, we conducted a multivariate analysis of the extracts tested.Initially, we assessed the correlation between the total bioactive compounds and the biological activities.As illustrated in Fig. 2A, the radical quenching and reducing potentials were strongly associated with these compounds.However, metal chelation and phosphomolybdenum capacities had no association with the total of phenolic and flavonoid components.This can be elucidated by the presence of non-phenolic substances like terpenoids or peptides.In agreement with our findings, several researchers highlighted a signficant relationship between the total bioactive constituents and antioxidant properties [68,69].In terms of enzyme inhibitory characteristics, no relationship was found with the total bioactive components.Principal component analysis was employed to demonstrate the similarity/dissimilarity among the tested samples and R 2 and O 2 that shows the fitness and predictive ability of the model were found as 0.98 and 0.82, respectively (Fig. 2B).In Fig. 2C, we observed a loading scatter plot of the tested variables, and the total bioactive components and antioxidant properties were the same in the plot.However, the enzyme inhibitory effects were classified in another plot.According to Fig. 2B and 2D, the tested extracts were classified into five groups.In comparison to the other extracts, the methanol extracts from both parts showed significantly stronger antioxidant activity, thereby setting them apart from the other extracts.At the same time, the chloroform extracts had a greater enzyme inhibition effect, thus leading them to be classified in the same group.It is clear that the plant parts and extraction solvents used influenced the distribution of extracts.Our findings can be utilized for further applications involving T. falconeri.
Four compounds exhibited higher binding affinities when docked against the ɑ-glucosidase enzyme, which attributes their contribution to the inhibitory effects of the plant extract.Murrayazolinine showed  the highest binding affinity due to -9.4 kcal binding energy compared to the standard inhibitor (acarbose; -8.3 kcal/mol) (Fig. 6), while the compounds kanzonol E (-9.1), N-acetyldehydroanonaine (-8.7), and castamollissin (-8.6) also showed better binding than acarbose.Moreover, kaempferol 3-p-coumarate depicted similar binding affinity to the standard and all the remaining compounds showed lesser affinity due to their higher binding energies (-5.8 to -8.2 kcal/mol) than the standard drug.
For tyrosinase inhibition only quinic acid (-5.3 kcal/ mol) showed lesser binding compared to the kojic acid (-5.4 kcal/mol) used as standard tyrosinase inhibitor.While, all the other docked ligands exhibited lower binding energies (-6.2 to -9.0 kcal/mol) showing their possible contribution in the tyrosinase inhibitory properties of the extract.The results further showed that kaempferol 3-p-coumarate has highest binding affinity towards tyrosinase enzyme due to its lowest binding energy (-9.0 kcal/mol) (Fig. 7).

ADME Analysis
The information anticipated for the medicinal chemistry, pharmacokinetics, lipophilicity, physicochemical properties, solubility, and drug resemblance of compounds assessed by SwissADME [70] is provided in Table 6.The molecular weights of the docked compounds were found within the range of 200-600 Da and to be 192.17-504.44,based on Lipinski's rule of five.The logP values were between -0.12 to 4.42, which were less than 5.All of the compound's products had an HBA number of 2 to 9 except for two compounds 6-caffeoylsucrose and castamollissin    which can accept 14 and 13 HBA respectively.While the same trend was observed for HBD numbers the two compounds have HBD numbers 9 and 8 respectively while the remaining compounds have HBD numbers ≤ 5 [71].The graphical model known as the Brain Or IntestinaL EstimateD Permeation (BOILED-Egg) technique determines the polarity and lipophilicity of small compounds.Concerning the possibility of oral absorption of medication candidates, this prediction offers a visual cue for the synthesis design of novel compounds [72].Figure 8 displays a graphic estimate of these selectively docked compounds' gastrointestinal absorption and blood-brain barrier (BBB) penetration.The compounds N-Acetyldehydroanonaine, Purpuritenin A, Murrayazolinine, Rutacultin, 9-Acetoxyfukinanolide and Matteucinol were found in the BBB, while Bryaflavan, Brosimacutin C, Methylsyringin and Kanzonol E were found in white region.The white region contains those compounds which have good potential to be absorbed through the gastrointestinal tract.The compounds 3-O-Feruloylquinic acid, Quinic acid and Kaempferol 3-p-coumarate as indicated by the BOILED-Egg plot were presented in gray region.The gray region is designated for poor intestine absorption.The two compounds 6-caffeoylsucrose and castamollissin violated Lipinski's rule and were not shown in BOILED-Egg The compounds showed with blue spot, was discovered to be indicative of their high bioavailability.The compounds Bryaflavan, Brosimacutin C, Methylsyringin and Kanzonol E show great promise as gastrointestinal tract absorbers since they do not cross the blood-brain barrier.These substances have no adverse effects on central nervous system depression or sleepiness because they cannot penetrate the blood-brain barrier.
TFFM: methanolic extract of flower of T. falconeri; TFFH: hexane extract of flower of T. falconeri; TFFE: ethyl acetate extract of flower of T. falconeri; TFFC: chloroform extract of flower of T. falconeri; TFSM: methanolic extract of stem of T. falconeri; TFSH: hexane extract of stem of T. falconeri; TFSE: ethyl acetate extract of stem of T. falconeri; TFSC: chloroform extract of stem of T. falconeri.Different letters in same column indicate significant differences in the tested extracts (p < 0.05) TFFM: methanolic extract of flower of T. falconeri; TFFH: hexane extract of flower of T. falconeri; TFFE: ethyl acetate extract of flower of T. falconeri; TFFC: chloroform extract of flower of T. falconeri; TFSM: methanolic extract of stem of T. falconeri; TFSH: hexane extract of stem of T. falconeri; TFSE: ethyl acetate extract of stem of T. falconeri; TFSC: methanolic extract of stem of T. falconeri.Different letters in same column indicate significant differences in the tested extracts (p < 0.05)

Fig. 3
Fig. 3 Diagrammatic presentation of 2D (left) and 3D (right) interactions of Kanzonol E with acetylcholinesterase active site residues

Table 2
Antioxidant activities of the extracts of T. falconeri respectively.Highest metal chelating activity was found for stem extracts, since TFSC and TFSM displayed chelating power as 18.06 ± 0.61 and 15.57 ± 0.22 mg TE/g extract, whereas, flower extracts were found weak chelators (Table TFFM: methanolic extract of flower of T. falconeri; TFFH: hexane extract of flower of T. falconeri; TFFE: ethyl acetate extract of flower of T. falconeri; TFFC: chloroform extract of flower of T. falconeri; TFSM: methanolic extract of stem of T. falconeri; TFSH: hexane extract of stem of T. falconeri; TFSE: ethyl acetate extract of stem of T. falconeri; TFSC: methanolic extract of stem of T. falconeri.Different letters in same column indicate significant differences in the tested extracts (p < 0.05)

Table 3
Enzyme inhibition activities of the extracts of T. falconeri

Table 4
UHPLC-MS analysis of methanolic extract of flower of T. falconeri

Table 5
Binding energies of identified compounds after docking with enzymesStandards: Galantamine (a), acarbose (b), and kojic acid (c).While AChE and BChE represent the acetylcholinesterase and butyrylcholinesterase respectively

Table 6
Medicinal and Drug-like properties of selected compounds predicted using SwissADME